HLA loss STR FA Kit
The test kit is intended for qualitative analysis of STR polymorhism fir the HLA loss detection by using capillary electrophoresis based on fragment analysis
Additional information about the molecular marker
One of the factors that can impact the success of BMT is the degree of human leukocyte antigen (HLA) matching between the donor and recipient. HLA molecules are expressed on the surface of all nucleated cells in the body and play a critical role in immune recognition and response. In BMT, the donor's immune system recognizes the recipient's cells as foreign and attacks them, leading to GVHD. HLA matching helps to minimize this risk.
However, even with HLA-matched donors, loss of HLA expression on recipient cells can occur, leading to relapse of the underlying disease. This loss of HLA expression can be caused by a variety of mechanisms, including genetic mutations and epigenetic changes.
One approach to detecting HLA loss is short tandem repeat (STR) analysis at chromosome 6p. This method involves amplifying the DNA in the region of chromosome 6p that contains the HLA genes and performing PCR with fluorescently labeled primers that target specific STR markers. The resulting PCR products are then analyzed by capillary electrophoresis to determine the size and quantity of each fragment.
By comparing the STR profiles of pre-transplant and post-transplant samples from the recipient, it is possible to detect changes in the HLA genotype that may indicate loss of HLA expression. This information can be used to guide treatment decisions, such as the use of donor lymphocyte infusions or other immunotherapies.
Several studies have demonstrated the utility of STR analysis for detecting HLA loss in BMT recipients. In particular a study published in Blood in 2017 found that STR analysis at chromosome 6p detected HLA loss in 44% of relapsed BMT patients, compared to only 20% using conventional cytogenetic analysis.
In conclusion, STR analysis at chromosome 6p is a valuable tool for detecting HLA loss in BMT recipients, which can inform treatment decisions and improve patient outcomes. Further research is needed to optimize the sensitivity and specificity of this method and to determine its utility in other contexts, such as solid organ transplantation and cancer immunotherapy.
Polymerase chain reaction (PCR) should be performed by solid-state PCR thermocyclers by any manufacturer, but the following PCR conditions are adapted for Bio-Rad cyclers.
The Life Technologies (3130, 3500, 3500xl) genetic analysers with POP7 polymer are recommended for capillary gel electrophoresis. GeneMapper, Peak Scanner, or GeneMarker software may be used for the fragment analysis
Publications:
1. Robert Zeiser, Luca Vago; Mechanisms of immune escape after allogeneic hematopoietic cell transplantation. Blood 2019; 133 (12): 1290–1297.
Ordering information:
HLA loss STR FA Kit, 24 tests Cat.No FA-7-24
Additiional reagents:
Blood Column DNA Kit, 100 extr. Cat.No CDK-100
Price: on request
Reagents are for research use only (RUO)